Our organoid and immune cell co-culture platform can be used to evaluate new immunomodulatory compounds such as bi-specifics, checkpoint inhibitors, or CAR-T cells. Our standardized assay format allows to measure reactivity of high-dose-IL-2 expanded, unbiased/polyclonal Tumor-Infiltrating Lymphocytes (TIL) against paired cancer patient-derived HUB Organoids™. TILs reactivity is measured by quantifying the level of induced tumor organoid cell death. If paired TIL are not available, non-paired T cell clones and HLA-matched peptide pulsed organoids can be exploited.
Assay layout: 96-well plate format
Read out: organoid cell death and/or IFNγ production
Application area: immuno-oncology
Assay validated on the following HUB Organoid™ models
Cytokine analysis by ELISA
Flow cytometry for phenotyping and to assess TIL viability
On request, we can set up specific assays that are not listed and which we have not yet developed.