The forskolin-induced swelling (FIS) assay is an industry standard assay used to quantify Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) protein activity, which is hampered in Cystic Fibrosis (CF) patients. At HUB we have validated the use of our CF patient-derived organoids in forskolin-induced swelling assay for the evaluation of new treatment strategies. Activation of CFTR by forskolin leads to chloride and subsequent water influx into the organoid lumen. The resulting FIS of the organoid can be measured by time-lapse confocal microscopy. Results are presented as normalized organoid swelling curves over time. The area under the curve (AUC) is determined as a measure for CFTR activity.
Assay layout: 96-well plate format
Read out: quantification of organoid forskolin-induced swelling (FIS)
Application area: CF
Assay validated on the following HUB Organoid™ models
Cytokine secretion by ELISA
On request, we can set up specific assays that are not listed and which we have not yet been developed.